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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | L6C5 |
| Accession number | ICLC AL00001 |
| Brief description | Species: rat; Tissue: skeletal muscle |
| Description | Species: rat newborn; Tissue: skeletal muscle; Derived from: L6 cell line (ATCC CRL 1458) |
| Depositor | Dr S. Adamo, Medical Istology Embriology Dept, University La Sapienza, Rome, Italy |
| Reference paper | Am J Physiol 1993;265:C113-C121 - DOI: 10.1152/ajpcell.1993.265.1.C113 - PMID: 8393277 |
| Morphology and growth | continuous culture, grown as monolayer, morphology myoblast |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | Myogenic, vasopressin responsive. Differentiation is induced by reduction of serum, IGF-1, vasopressin (AVP). AVP stimulates PLC, PLD, cAMP-phosphodiesterase. Skeletal muscle myosin, creatine kinase, myogenin, vasopressin receptor V1a, IGF-1 receptor, nicotinic acetylcholine receptor subunits. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as rat with MD, NP |
| Passage number | 10 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Cell Growth Diff 1995;6:81-89 - PMID: 7718487
Cell Growth Diff 1998;9:155-164 - PMID: 9486852 J Cell Physiol 1997;171:34-42 - DOI: 10.1002/(SICI)1097-4652(199704)171:1<34::AID-JCP5>3.0.CO;2-I - PMID: 9119890 Lipids 1999;34:581-582 Mol Biol Cell 1999;10(12)in press |
| Comments | L6C5 fuse in culture to form multinucleated myotubes. The myogenic potential is lost if cells are allowed to become confluent. |