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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | BRL-3A |
| Accession number | ICLC AL00002 |
| Brief description | Species: rat, buffalo; Tissue: liver |
| Description | Species: rat, buffalo female 5 weeks old; Tissue: liver |
| Depositor | Dr T. Russo, Biochemistry and Medical Biotechnology Dept, Naples, Italy |
| Reference paper | Carnegie Inst. Washington Yearbook 1968;67:419-421 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | Coon's F12 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:10-1:20 using trypsin/EDTA; seed at 1-3 x 10^4 cells/cm2; 37C, 5%CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as rat with NP, PepB |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Cell 1977;11:441-446 - DOI: 10.1016/0092-8674(77)90062-9
- PMID: 302146
Proc Natl Acad Sci USA 1969;62:852-859 - DOI: 10.1073/pnas.62.3.852 - PMID: 4308097 |