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Cell line nameCTLL-2
Accession numberICLC AL00003
Brief descriptionSpecies: mouse, C57BL/6; Tissue: lymphocyte, T
DescriptionSpecies: mouse, C57BL/6; Tissue: lymphocyte, T; Derived from: cytotoxic T cells
DepositorDr S. Ferrini, Immunopharmacolgy Dept, IST at ABC, Genoa, Italy
Reference paperNature 1977;268:154-156 - PMID: 145543
Morphology and growthcontinuous culture, grown in suspension, morphology single cells and few small clusters
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine + 50 u/ml IL-2 + 4.7 x 10^-5M 2-mercaptoethanol; maintain cultures between 3x10^4 and 1x10^5 cells/ml; split 1:5-1:20. 37C, 5% CO2
PropertiesThe cells are dependent on Interleukin-2 (IL-2) for growth and are used as an assay system for this factor
DistributionCell line available for distribution, only as frozen ampoule. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as mouse with MD, MPI
Freezing medium90% FBS + 10% DMSO
CommentsAfter thawing the viability of the cells may be very low. Thaw the cells with medium with 20% FBS and seed in 24-well-plates at density of 2-6 x 10^5 cells/ml.

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