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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | CTLL-2 |
| Accession number | ICLC AL00003 |
| Brief description | Species: mouse, C57BL/6; Tissue: lymphocyte, T |
| Description | Species: mouse, C57BL/6; Tissue: lymphocyte, T; Derived from: cytotoxic T cells |
| Depositor | Dr S. Ferrini, Immunopharmacolgy Dept, IST at ABC, Genoa, Italy |
| Reference paper | Nature 1977;268:154-156 - DOI: 10.1038/268154a0 - PMID: 145543 |
| Morphology and growth | continuous culture, grown in suspension, morphology single cells and few small clusters |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine + 50 u/ml IL-2 + 4.7 x 10^-5M 2-mercaptoethanol; maintain cultures between 3x10^4 and 1x10^5 cells/ml; split 1:5-1:20. 37C, 5% CO2 |
| Properties | The cells are dependent on Interleukin-2 (IL-2) for growth and are used as an assay system for this factor |
| Distribution | Cell line available for distribution, only as frozen ampoule. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with MD, MPI |
| Freezing medium | 90% FBS + 10% DMSO |
| Comments | After thawing the viability of the cells may be very low. Thaw the cells with medium with 20% FBS and seed in 24-well-plates at density of 2-6 x 10^5 cells/ml. |