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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | NIH 3T3 |
| Accession number | ICLC AL07001 |
| Brief description | Species: mouse, NIH Swiss; Tissue: embryo |
| Description | Species: mouse, NIH Swiss; Tissue: embryo |
| Depositor | Dr. R. P. Revoltella, Fondazione ONLUS - Staminali e Vita - Pisa, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; split 1:5 - 1:10, 37C, 5% CO2 |
| Properties | used in DNA transfection studies ; Susceptible to: murine leukemia virus, murine sarcoma virus |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Passage number | 120 |
| Freezing medium | Culture medium + 5% DMSO |
| Further bibliography | J Virol. 1969;4(5): 549-553, - DOI: 10.1128/JVI.4.5.549-553.1969
- PMID: 4311790
Cell 1979;16(1): 63-75 - DOI: 10.1016/0092-8674(79)90188-0 - PMID: 84715 Cell 1979;16(2): 347-356 - DOI: 10.1016/0092-8674(79)90011-4 - PMID: 222457 |
| Comments | Cell line is susceptible to murine sarcoma virus and murine leukemia virus |