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IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
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Cell line nameSIRC
Accession numberICLC AL96001
Brief descriptionSpecies: rabbit; Tissue: cornea
DescriptionSpecies: rabbit; Tissue: cornea
DepositorDr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy.
Reference paperProc Soc Exp Biol Med 1970;135:1543-1545
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsMEM (EBSS) + 10% FBS + 1% non-essential amino acids; Split confluent cultures 1:4-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2.
Propertiesvirus replication; not susceptible to poliovirus2, Coxsackie A9 and B5; Susceptible to: rubella
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as rabbit with AST, G6PD, PepB
Passage number12
Karyologymodal number 66
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyProc Soc Exp Biol Med 1966;122:783 - DOI: 10.3181/00379727-122-31251 - PMID: 5918951
Proc Soc Exp Biol Med 1967;125:1271 - DOI: 10.3181/00379727-125-32332 - PMID: 6042441
Virology 1984;132:79-84 - DOI: 10.1016/0042-6822(84)90093-x - PMID: 6607566

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