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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SIRC |
| Accession number | ICLC AL96001 |
| Brief description | Species: rabbit; Tissue: cornea |
| Description | Species: rabbit; Tissue: cornea |
| Depositor | Dr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy. |
| Reference paper | Proc Soc Exp Biol Med 1970;135:1543-1545 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
| Culture conditions | MEM (EBSS) + 10% FBS + 1% non-essential amino acids; Split confluent cultures 1:4-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2. |
| Properties | virus replication; not susceptible to poliovirus2, Coxsackie A9 and B5; Susceptible to: rubella |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as rabbit with AST, G6PD, PepB |
| Passage number | 12 |
| Karyology | modal number 66 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Proc Soc Exp Biol Med 1966;122:783 - DOI: 10.3181/00379727-122-31251
- PMID: 5918951
Proc Soc Exp Biol Med 1967;125:1271 - DOI: 10.3181/00379727-125-32332 - PMID: 6042441 Virology 1984;132:79-84 - DOI: 10.1016/0042-6822(84)90093-x - PMID: 6607566 |