Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | Mv1Lu |
Accession number | ICLC AL97002 |
Brief description | Species: mustela vison (mink); Tissue: lung, fetal |
Description | Species: mustela vison (mink); Tissue: lung, fetal |
Depositor | Dr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy. |
Reference paper | Unknown |
Morphology and growth | monoclonal continuous culture, grown as monolayer, morphology fibroblast-like |
Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2. |
Properties | virology; Susceptible to: vaccinia, herpes simplex, reovirus 3, vesicular stomatitis (Ogden strain) |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by isoenzymes: confirmed as mink with MD, Pep B |
Passage number | 40 |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Further bibliography | In Vitro 1983;19:7 - DOI: 10.1007/BF02617988
- PMID: 6337087
Virology 1974;60:282-287 - DOI: 10.1016/0042-6822(74)90386-9 - PMID: 4366800 Virology 1975;67:344 - DOI: 10.1016/0042-6822(75)90436-5 - PMID: 52940 |