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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | Cf2Th |
| Accession number | ICLC AL97003 |
| Brief description | Species: dog; Tissue: thymus |
| Description | Species: dog; Tissue: thymus |
| Depositor | Dr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy. |
| Reference paper | In Vitro 1976;12:665 - DOI: 10.1007/BF02797468 - PMID: 190163 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2 |
| Properties | Permissive for xenotropic viruses found in Balb/c and AKR mice; C-type viruses growth; Susceptible to: reovirus, pseudorabies, herpes simplex, parvovirus, calicivirus, feline rhinotracheitis, retroviruses |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as dog with G6PD, MD. |
| Passage number | 9 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Am J Vet Res 1983;44:865 - PMID: 6869994
In Vitro 1977;9:176 - DOI: 10.1016/0006-291x(75)90293-4 |