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Cell line nameE.Derm
Accession numberICLC AL97004
Brief descriptionSpecies: horse; Tissue: dermis
DescriptionSpecies: horse female 4 years old; Tissue: dermis
DepositorDr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsMEM (EBSS) + 10% FBS + 1% non-essential amino acids; Split confluent cultures 1:2-1:3 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2. Finite lifespan of approximately 40 passages.
PropertiesSusceptible to: herpes simplex, reovirus 3, coronavirus, equine infectious anaemia virus, vesicular stomatitis (Ogden strain)
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as horse with NP
Passage number20
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyAm J Vet Res 1981;42:1501-1505 - PMID: 6275755
Am J Vet Res 1986;47:1931-1934 - PMID: 3021027
Int J Cancer 1975;15:171-179 - PMID: 165152

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