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Cell line nameV-79
Accession numberICLC AL99002
Brief descriptionSpecies: hamster, Chinese; Tissue: lung, fibroblast
DescriptionSpecies: hamster, Chinese male; Tissue: lung, fibroblast
DepositorDr A. Stammati, Dept of Toxicology, ISS, Rome, Italy
Reference paperJ Natl Cancer Inst 1958:21:393-425
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast
Culture conditionsDMEM + 5% FBS + 2 mM L-Glutamine; split confluent cultures 1:10-1:20 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2
Propertiesthe cells have been used for studies on the G1 phase of the cell cycle
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as hamster with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyMutagenesis 1990;5:241-249 - PMID: 2385178
Nature 1959;184:1293-1295
Commentsthe cells detach very easily (1-2 min.)

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