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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | V-79 |
| Accession number | ICLC AL99002 |
| Brief description | Species: hamster, Chinese; Tissue: lung, fibroblast |
| Description | Species: hamster, Chinese male; Tissue: lung, fibroblast |
| Depositor | Dr A. Stammati, Dept of Toxicology, ISS, Rome, Italy |
| Reference paper | J Natl Cancer Inst 1958:21:393-425 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast |
| Culture conditions | DMEM + 5% FBS + 2 mM L-Glutamine; split confluent cultures 1:10-1:20 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2 |
| Properties | the cells have been used for studies on the G1 phase of the cell cycle |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as hamster with MD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Mutagenesis 1990;5:241-249 - DOI: 10.1093/mutage/5.3.241
- PMID: 2385178
Nature 1959;184:1293-1295 |
| Comments | the cells detach very easily (1-2 min.) |