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Cell line nameL6H2
Accession numberICLC ATL00001
Brief descriptionSpecies: rat; Transformed by: non myogenic subclone; Original line: L6 (ATCC CRL1458)
DescriptionSpecies: rat newborn; spontaneously transformed; non myogenic subclone of the rat myogenic L6 cell line; Original line: L6 (ATCC CRL1458)
DepositorDr S. Adamo, Medical Istology Embriology Dept, University La Sapienza, Rome, Italy
Reference paperAm J Physiol 1993;265:C113-C121 - PMID: 8393277
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like and round cells lightly adherent
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split cultures 1:10-1:20 using Trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
PropertiesL6H2 display limited adhesion, do not give rise to myotubes, grow in soft agar
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as rat with MD, NP
Passage number7
Freezing mediumCulture medium + 50% FBS + 10% DMSO

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