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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | McA-RH7777 |
| Accession number | ICLC ATL00002 |
| Brief description | Species: rat, buffalo; Tissue: liver; Tumor: hepatoma |
| Description | Species: rat, buffalo; Tissue: liver; Tumor: hepatoma; Derived from: transplantable Morris hepatoma 7777, induced by N-2 fluorenylphthalamic acid |
| Depositor | Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy |
| Reference paper | Recent Results Cancer Res 1974;44:103-114 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + Ham's F12 (1:1) + 7-10% FBS + 2 mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-3 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | albumin and alpha fetoprotein production |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as rat with MD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Onco-Developmental Gene Expression, pp. 259-270, Fishman and Sell eds., Academic Press, New York, 1976 |
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