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Cell line nameMcA-RH7777
Accession numberICLC ATL00002
Brief descriptionSpecies: rat, buffalo; Tissue: liver; Tumor: hepatoma
DescriptionSpecies: rat, buffalo; Tissue: liver; Tumor: hepatoma; Derived from: transplantable Morris hepatoma 7777, induced by N-2 fluorenylphthalamic acid
DepositorProf. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Reference paperRecent Results Cancer Res 1974;44:103-114
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + Ham's F12 (1:1) + 7-10% FBS + 2 mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-3 x 10^4 cells/cm2; 37C, 5% CO2
Propertiesalbumin and alpha fetoprotein production
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as rat with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyOnco-Developmental Gene Expression, pp. 259-270, Fishman and Sell eds., Academic Press, New York, 1976
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