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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | COS-1 |
| Accession number | ICLC ATL01002 |
| Brief description | Species: monkey, African green; Tissue: kidney; Transformed by: SV40 origin defective mutant; Original line: CV-1 |
| Description | Species: monkey, African green; Tissue: kidney; Transformed by: SV40 origin defective mutant; Original line: CV-1 |
| Depositor | Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy. |
| Reference paper | Cell 1981;23:175-182 - DOI: 10.1016/0092-8674(81)90282-8 - PMID: 6260373 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:4-1:10 using trypsin/EDTA; 37C, 5% CO2 |
| Properties | This line contains T antigen. Suitable for transfection by vectors requiring expression of SV40 T antigen |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | PCR analysis: confirmed as African green monkey with cytochrome c oxidase subunit I (cox I) primers |