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Cell line nameCOS-1
Accession numberICLC ATL01002
Brief descriptionSpecies: monkey, African green; Tissue: kidney; Transformed by: SV40 origin defective mutant; Original line: CV-1
DescriptionSpecies: monkey, African green; Tissue: kidney; Transformed by: SV40 origin defective mutant; Original line: CV-1
DepositorDr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy.
Reference paperCell 1981;23:175-182 - PMID: 81162716
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:4-1:10 using trypsin/EDTA; 37C, 5% CO2
PropertiesThis line contains T antigen. Suitable for transfection by vectors requiring expression of SV40 T antigen
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysPCR analysis: confirmed as African green monkey with cytochrome c oxidase subunit I (cox I) primers

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