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Cell line nameRAW 264.7
Accession numberICLC ATL02001
Brief descriptionSpecies: mouse, BALB/c; Tissue: macrophage
DescriptionSpecies: mouse, BALB/c male; Tissue: macrophage; Derived from: tumor induced by Abelson murine leukemia virus
DepositorDr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese.
Reference paperCell 1978;15:261-267 - DOI: 10.1016/0092-8674(78)90101-0 - PMID: 212198
Morphology and growthcontinuous culture, grown semiadherent, morphology macrophage
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split subconfluent cultures 1:5-1:10 using trypsin/EDTA or scraper; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysMultiplex PCR: tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyJ Immunol 1977;119:950-954 - PMID: 894031
CommentsThe cells grow adherent, but some are loosely attached to the surface and others in suspension

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