 |
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
|
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
| Cell line name | RAW 264.7 |
| Accession number | ICLC ATL02001 |
| Brief description | Species: mouse, BALB/c; Tissue: macrophage |
| Description | Species: mouse, BALB/c male; Tissue: macrophage; Derived from: tumor induced by Abelson murine leukemia virus |
| Depositor | Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese. |
| Reference paper | Cell 1978;15:261-267 - DOI: 10.1016/0092-8674(78)90101-0 - PMID: 212198 |
| Morphology and growth | continuous culture, grown semiadherent, morphology macrophage |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Split subconfluent cultures 1:5-1:10 using trypsin/EDTA or scraper; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Multiplex PCR: tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | J Immunol 1977;119:950-954 - PMID: 894031
|
| Comments | The cells grow adherent, but some are loosely attached to the surface and others in suspension |