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Cell line nameBV-2
Accession numberICLC ATL03001
Brief descriptionSpecies: mouse, C57BL/6; Tissue: brain, microglial cells; Transformed by: recombinant retrovirus (v-raf/v-mic)
DescriptionSpecies: mouse, C57BL/6; Tissue: brain, microglial cells; Transformed by: recombinant retrovirus (v-raf/v-mic)
DepositorDr E. Blasi, Dip. Scienze Igienistiche, Microbiologiche e Biostatistiche, Modena, Italy
Reference paperJ Neuroimmunol 1990;27:229-237 - PMID: 2110186
Morphology and growthcontinuous culture, grown semiadherent, morphology microglial
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split cultures 1:5-10; 37C, 5% CO2
PropertiesThe BV-2 cells express the nuclear v-myc and the cytoplasmic v-raf oncogene products as well as the env gp70 antigen at the surface level; the BV-2 cells have morphological, phenotypical and functional markers of macrophages
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle under Biosafety Level 2 containment. The BV-2 cell line produces an enveloped recombinant ecotropic retrovirus (capable of infecting murine cells only); such virus is known for its in vitro transforming ability and in vivo tumorigenic potential
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysMultiplex PCR: tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyCell Immunol 1996;170:251-259 - PMID: 8660825
Infect Immunol 1992;60:3682-3688 - PMID: 1500177
J Neuroimmunol 1991;34:53-60 - PMID: 1894734
J Neuroimmunol 1995;58:111-116 - PMID: 7730446
J Neuroimmunol 1998;93:102-107 - PMID: 10378873
J Neurosci Res 1992;31:616-621 - PMID: 1578513
Neuroimmunol 1991;32:249-257 - PMID: 2033118
CommentsThe cells grow loosely attached and in suspension

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