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Cell line nameNULLI-SCC1
Accession numberICLC ATL06001
Brief descriptionSpecies: mouse, 129/SV; Tissue: testis; Tumor: teratocarcinoma
DescriptionSpecies: mouse, 129/SV male; Tissue: testis; Tumor: teratocarcinoma
DepositorDr S. Astigiano, Transgenic Animals Dept, IST at ABC, Genoa, Italy
Reference paperCell 1975 ;5(3):229-43 - PMID: 1097123
Morphology and growthcontinuous culture, grown as monolayer, morphology undifferentiated
Culture conditionsDMEM (with 4.5 g/l glucose) + 10% Calf serum; split 1:5 - 1:10, 3 times per week; 37C, 5% CO2
Propertiespluripotent; differentiation
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Freezing medium95% FCS + 5% DMSO
Validation assaysMultiplex PCR: tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers
CommentsGrowth on gelatin-coated dishes. Differentiation into visceral or parietal endoderm with retinoic acid or HMBA treatment

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