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Cell line nameMTP-GFP
Accession numberICLC ATL07002
Brief descriptionSpecies: mouse, CD-1 transgenic; Tissue: mammary gland; Tumor: adenocarcinoma; Transformed by: MMTVneu oncogene
DescriptionSpecies: mouse, CD-1 transgenic female; Tissue: mammary gland; Tumor: adenocarcinoma; Transformed by: MMTVneu oncogene; Derived from: lung metastasis
DepositorDr M.G. Sacco, Human Genome Dept, ITB-CNR, Milan, Italy
Reference paperCell Prolif. 2006 Dec;39(6):611-22. - PMID: 17109643
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsWilliam's medium + 15% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:2-1:4; 37C, 5% CO2
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative
Hazard
Passage number19
Freezing mediumCulture medium + 10% FBS + 10% DMSO
Validation assaysMultiplex PCR: tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers
Commentsfluorescent cells. Cells also grow well with the heat inactivated serum

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