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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MTP-GFP |
| Accession number | ICLC ATL07002 |
| Brief description | Species: mouse, CD-1 transgenic; Tissue: mammary gland; Tumor: adenocarcinoma; Transformed by: MMTVneu oncogene |
| Description | Species: mouse, CD-1 transgenic female; Tissue: mammary gland; Tumor: adenocarcinoma; Transformed by: MMTVneu oncogene; Derived from: lung metastasis |
| Depositor | Dr M.G. Sacco, Human Genome Dept, ITB-CNR, Milan, Italy |
| Reference paper | Cell Prolif. 2006 Dec;39(6):611-22. - DOI: 10.1111/j.1365-2184.2006.00401.x - PMID: 17109643 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | William's medium + 15% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:2-1:4; 37C, 5% CO2 |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative |
| Hazard | |
| Passage number | 19 |
| Freezing medium | Culture medium + 10% FBS + 10% DMSO |
| Validation assays | Multiplex PCR: tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers |
| Comments | fluorescent cells. Cells also grow well with the heat inactivated serum |