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Cell line nameSHM-D33
Accession numberICLC ATL94001
Brief descriptionSpecies: mouse / human; Tissue: hybrid; Tumor: heteromyeloma
DescriptionSpecies: mouse / human; Tissue: hybrid; Tumor: heteromyeloma
DepositorDr V. Dessi, San Martino Hospital, Genoa, Italy.
Reference paperProc Natl Acad Sci U S A 1983;80:7308 - PMID: 6316357
Morphology and growthcontinuous culture, grown semiadherent, morphology lymphoblast
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; maintain cultures between 3 and 10x105 cells/ml; split 1:5-1:10; the cell line grows semiadherent.37C, 5% CO2.
Propertiesresistant to ouabain; fusion partner; hybridization; tumorigenicity
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: pattern compatible with mouse (Pep B, AST, NP)
Karyology83 murine chromosomes + 4 human chromosomes
Freezing mediumMEM + 20% FBS + 10% DMSO

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