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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SHM-D33 |
| Accession number | ICLC ATL94001 |
| Brief description | Species: mouse / human; Tissue: hybrid; Tumor: heteromyeloma |
| Description | Species: mouse / human; Tissue: hybrid; Tumor: heteromyeloma |
| Depositor | Dr V. Dessi, San Martino Hospital, Genoa, Italy. |
| Reference paper | Proc Natl Acad Sci U S A 1983;80:7308 - DOI: 10.1073/pnas.80.23.7308 - PMID: 6316357 |
| Morphology and growth | continuous culture, grown semiadherent, morphology lymphoblast |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; maintain cultures between 3 and 10x105 cells/ml; split 1:5-1:10; the cell line grows semiadherent.37C, 5% CO2. |
| Properties | resistant to ouabain; fusion partner; hybridization; tumorigenicity |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: pattern compatible with mouse (Pep B, AST, NP) |
| Karyology | 83 murine chromosomes + 4 human chromosomes |
| Freezing medium | MEM + 20% FBS + 10% DMSO |