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Cell line nameL929
Accession numberICLC ATL95001
Brief descriptionSpecies: mouse, C3H/An; Tissue: connective
DescriptionSpecies: mouse, C3H/An male 100 days old; Tissue: connective
DepositorObtained from ECACC.
Reference paperJ Natl Cancer Inst 1948;9:229
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at1x104 cells/cm2; 37C, 5% CO2.
Propertiesinterferon assay; virus titration; tumor necrosis factor assay; virology; Susceptible to: pseudorabies, herpes simplex, vesic. stomatitis (Indiana), bluetongue virus
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as mouse with AST, G6PD, MD, NP, Pep B
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyJ Clin Microbiol 1988;26:2324 - PMID: 2853175
J Natl Cancer Inst 1943;4:165-212

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