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Cell line name3T3
Accession numberICLC ATL95002
Brief descriptionSpecies: mouse, Swiss albino; Tissue: embryo
DescriptionSpecies: mouse, Swiss albino; Tissue: embryo
DepositorObtained from ECACC
Reference paperJ Cell Biol 1963;17:299-313
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2
PropertiesContact inhibited; transfection studies. The cell line is a valuable in vitro host system for oncogenic transformation studies. It is readily transformed by SV40 and polyoma viruses
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as mouse with G6PD, MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyVirology 1965;27:179-185 - PMID: 4284655

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