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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | 3T3 |
| Accession number | ICLC ATL95002 |
| Brief description | Species: mouse, Swiss albino; Tissue: embryo |
| Description | Species: mouse, Swiss albino; Tissue: embryo |
| Depositor | Obtained from ECACC |
| Reference paper | J Cell Biol 1963;17:299-313 - DOI: 10.1016/0006-291x(75)90293-4 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2 |
| Properties | Contact inhibited; transfection studies. The cell line is a valuable in vitro host system for oncogenic transformation studies. It is readily transformed by SV40 and polyoma viruses |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with G6PD, MD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Virology 1965;27:179-185 - DOI: 10.1016/0042-6822(65)90157-1
- PMID: 4284655
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