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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | CHO |
| Accession number | ICLC ATL95003 |
| Brief description | Species: hamster, Chinese; Tissue: ovary |
| Description | Species: hamster, Chinese female; Tissue: ovary |
| Depositor | Obtained from ECACC |
| Reference paper | J Exp Med 1958;108:945 - DOI: 10.1016/0006-291x(75)90293-4 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like and epithelial-like |
| Culture conditions | Ham's F12 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA;seed at 1-3x104 cells/cm2;37C, 5% CO2 |
| Properties | genetics; toxicity screening; nutrition; gene expression |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as hamster with AST, MD |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |