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Cell line nameVero
Accession numberICLC ATL95005
Brief descriptionSpecies: monkey, African green; Tissue: kidney
DescriptionSpecies: monkey, African green adult; Tissue: kidney
DepositorObtained from ECACC.
Reference paperNippon Rinsho 1963;21:1209 - DOI: 10.1016/0006-291x(75)90293-4
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:20 using trypsin/EDTA; seed at 1-3x104 cells/cm2. 37C, 5% CO2.
Propertiesindicator line for mycoplasma testing; virology; virus titration; virus replication; plaque assays; bacterial cytotoxicity.; Susceptible to: poliovirus 3, echovirus, reovirus, rubella, herpes simplex, arbovirus, adenovirus 12, influenza, paramyxovirus, Getah arbovirus, orthomixovirus, African swine fever virus, bluetongue virus, bovine leucosis, orbivirus, porcine epidemic diarrhea virus
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as monkey with AST, MD, NP, PepB
Karyologymodal number 58
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyKitasato Arc Exp Med 1964;37:27-42 - PMID: 5833688
Nature 1967;216:271 Proc Soc Exp Biol Med 1967;125:602 - DOI: 10.3181/00379727-125-32157 - PMID: 4961492

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