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Cell line nameC6
Accession numberICLC ATL95007
Brief descriptionSpecies: rat; Tumor: glial tumor
DescriptionSpecies: rat; Tumor: glial tumor
DepositorDr T. Florio, Neuroscience Dept, ABC, Genoa Italy.
Reference paperScience 1968;161:370 - PMID: 4873531
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsHam's F12 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-3 x 106 cells/cm2. 37C, 5% CO2
PropertiesS-100 protein production (depending on the level of confluence). HLA: B27.; Susceptible to: vaccinia, herpes simplex, vesic. stomatitis (Indiana)
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as rat with AST, MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyFed Proc 1968;27:720

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