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Cell line nameRT-1
Accession numberICLC ATL96002
Brief descriptionSpecies: rat, Lewis; Tumor: fibrosarcoma; Transformed by: RSV-33
DescriptionSpecies: rat, Lewis; Tumor: fibrosarcoma; Transformed by: RSV-33; tumor induced in vivo by RSV-33; Derived from: co-culture of rat embryonic fibroblasts with chicken sarcoma cells
DepositorObtained from ECACC.
Reference paperFolia Biol (Praha)1992;38:90-102 - PMID: 1326448
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsMEM (EBSS) + 5% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2. 37C, 5% CO2.
PropertiesThe cells contain the RSV-33 viral genome, but do not express virus specific RNA
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as rat with AST, NP, MD
Tumorigenicitytumorigenic in syngeneic animals
Karyologymodal chromosome number 40
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyNeoplasma 1991;38:617 - PMID: 1662779

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