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Cell line nameGH3
Accession numberICLC ATL96003
Brief descriptionSpecies: rat, Wistar-Furth; Tumor: pituitary tumor
DescriptionSpecies: rat, Wistar-Furth female 7 months old; Tumor: pituitary tumor
DepositorDr T. Florio, Neuroscience Dept, ABC, Genoa, Italy
Reference paperEndocrinology 1968;82:342 - DOI: 10.1210/endo-82-2-342 - PMID: 4951281
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsHam's F10 + 10% FBS + 2mM L-Glutamine; the cells are capable of adaptation in suspension culture using MEM. Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2.
PropertiesGrowth hormone, somatotropin and prolactin secretion.; Susceptible to: herpes simplex, vesic. stomatitis (Indiana)
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as rat with MD, MPI
Tumorigenicitytumorigenic in syngeneic animals
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyIn Vitro 1970;6:180 - DOI: 10.1007/BF02617762 - PMID: 5535574
J Cell Biol 1969;43:432 - DOI: 10.1083/jcb.43.3.432 - PMID: 5389137

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