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Cell line nameWEHI-164
Accession numberICLC ATL96004
Brief descriptionSpecies: mouse, BALB/c; Tumor: fibrosarcoma; Transformed by: tumor induced by subcutaneous injection of 3-methylcholanthrene
DescriptionSpecies: mouse, BALB/c; Tumor: fibrosarcoma; Transformed by: tumor induced by subcutaneous injection of 3-methylcholanthrene
DepositorObtained from ECACC.
Reference paperProc Soc Exp Biol Med 1973;144:813-818 - PMID: 4543620
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA, seed at 2-4 x 104 cells/cm2. 37C, 5% CO2.
Propertiestumorigenicity and cytotoxicity studies: highly sensitive to human cytotoxic monocytes, human tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) after pretreatment with actinomycin D.
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as mouse with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyBlood 1985;65:8-14 - PMID: 3155490
J Natl Cancer Inst 1984;72:23-29 - PMID: 6582301

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