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Cell line nameCOS-7
Accession numberICLC ATL96005
Brief descriptionSpecies: monkey, African green; Tissue: kidney; Transformed by: SV40origin defective mutant; Original line: CV-1
DescriptionSpecies: monkey, African green; Tissue: kidney; Transformed by: SV40origin defective mutant; Original line: CV-1
DepositorDr T. Florio, Neuroscience Dept, ABC, Genoa, Italy
Reference paperCell 1981;23:175-182 - PMID: 81162716
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2
PropertiesThe line contains T antigen. Suitable for transfection by vectors requiring expression of SV40 T antigen.
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as monkey with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO

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