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Cell line nameGH4-C1
Accession numberICLC ATL96006
Brief descriptionSpecies: rat, Wistar-Furth; Tumor: pituitary adenoma
DescriptionSpecies: rat, Wistar-Furth female 7 months old; Tumor: pituitary adenoma
DepositorDr T. Florio, Neuroscience Dept, ABC, Genoa, Italy.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsHam's F10 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2 37C, 5% CO2.
PropertiesThe cells were used for studies on growth hormon and prolactin secretion.
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as rat with AST, G6PD, PepB
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyJ Gen Physiol 1984;83:309-339 - DOI: 10.1085/jgp.83.3.309 - PMID: 6325585
Proc Natl Acad Sci USA 1985;82:4282-4286 - DOI: 10.1073/pnas.82.12.4282 - PMID: 2408278
CommentsAfter thawing the viability of the cells may be low

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