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Cell line nameC2-Rev7
Accession numberICLC ATL96007
Brief descriptionSpecies: rat; Tumor: hepatoma
DescriptionSpecies: rat; Tumor: hepatoma
DepositorObtained from ECACC.
Reference paperCell 1980;19:1043 - DOI: 10.1016/0092-8674(80)90095-1 - PMID: 6247070
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsCoon's modified Ham's F12 + 5% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 9x103 cells/cm2. 37C, 5% CO2.
PropertiesHGPRT negative
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as rat with AST, Pep B
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyEur J Biochem 1984;145:455-462 - DOI: 10.1111/j.1432-1033.1984.tb08577.x - PMID: 6334605

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