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Cell line nameS+L-CAT2
Accession numberICLC ATL96011
Brief descriptionSpecies: cat; Transformed by: murine sarcoma virus
DescriptionSpecies: cat; Transformed by: murine sarcoma virus
DepositorDr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsRPMI 1640 or DMEM + 2 mM L-Glutamine + 10% FBS; Split 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2. 37C, 5% CO2.
Propertiesvirus replication; virology; the cell line does not produce murine leukemia virus (L-).
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as cat with AST, G6PD
Passage number7
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyJ Gen Virol 1983;64:425-428 - DOI: 10.1099/0022-1317-64-2-425 - PMID: 6300292
J Virol 1974;14:177-179 - DOI: 10.1128/JVI.14.1.177-179.1974 - PMID: 4365331
Virology 1975;67:288-291 - DOI: 10.1016/0042-6822(75)90427-4 - PMID: 170737

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