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Cell line nameP3X63Ag8U.1
Accession numberICLC ATL96012
Brief descriptionSpecies: mouse, BALB/c; Tumor: myeloma
DescriptionSpecies: mouse, BALB/c female; Tumor: myeloma; Derived from: P3X63Ag8
DepositorObtained from ECACC.
Reference paperCurr Top Microbiol Immunol 1978;81:1-7 - PMID: 567551
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split 1:5-1:10; maintain cultures between 3 and 9x105 cells/ml; rapid cell death occurs at >1x106 cells/ml. 37C, 5% CO2.
Propertiesfusion partner; HGPRT- and HAT sensitive; does not synthesize gamma1 chains, produces kappa light chains which are not secreted
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as mouse with AST, G6PD
Freezing mediumCulture medium + 50% FBS + 10% DMSO

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