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Cell line nameXC
Accession numberICLC ATL96013
Brief descriptionSpecies: rat, Wistar; Tumor: sarcoma; Transformed by: Rous sarcoma virus
DescriptionSpecies: rat, Wistar; Tumor: sarcoma; Transformed by: Rous sarcoma virus; tumor induced by intramuscular injection
DepositorDr O.E. Varnier, Molecular Virology Dept, ABC, Genoa, Italy.
Reference paperFolia Biol (Praha) 1962;8:221
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMEM (HBSS) + 10% FBS + 2 mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-5x104 cells/cm2. 37C, 5% CO2.
Propertiesvirology; plaque assays; used as indicator cells for detection of murine leukemia viruses; Susceptible to: Moloney-MuLV, vesic. stomatitis (Indiana)
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as rat with AST, MD
Passage number42
Karyologyhypodiploid
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyChromosoma 1970;31:51-60 - PMID: 4321325
Nature 1960;186:980-981 Proc Natl Acad Sci U S A 1969;63:753-758 - PMID: 4186808
Virology 1970;42:1136-1139 - PMID: 4099080

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