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Cell line nameMiCl1 (S+L-)
Accession numberICLC ATL97001
Brief descriptionSpecies: mustela vison (mink); Tissue: lung, fetal; Transformed by: Moloney-MSV; Original line: Mv.1.Lu.
DescriptionSpecies: mustela vison (mink); Tissue: lung, fetal; Transformed by: Moloney-MSV; Original line: Mv.1.Lu.
DepositorDr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy.
Reference paperVirology 1975;67:288 - PMID: 170737
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA, see at 2-4x104 cells/cm2; 37C, 5% CO2.
VirusesMoloney-MSV
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as mink with MD, Pep B
Passage number13
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyScience 1976;192:1143 - PMID: 179144

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