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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | PG-4 (S+L-) |
| Accession number | ICLC ATL97002 |
| Brief description | Species: cat; Tissue: brain, fetal; Transformed by: Moloney-MSV; Original line: G355 |
| Description | Species: cat; Tissue: brain, fetal; Transformed by: Moloney-MSV; Original line: G355 |
| Depositor | Obtained from ECACC. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology glial/astrocyte |
| Culture conditions | McCoy's 5A + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. |
| Properties | virus replication; viral diagnostics; the cells can detect and assay type C retroviruses and are currently recommended by the FDA for detection of replication competent retroviruses in gene therapy products and hybridomas producing reagents for human use |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as cat with G6PD, MD. |
| Passage number | 26 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | J Virol 1985;53:827-833 - DOI: 10.1128/JVI.53.3.827-833.1985
- PMID: 2983093
Virology 1982;123:139-151 - DOI: 10.1016/0042-6822(82)90301-4 - PMID: 6959413 |