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Cell line namePG-4 (S+L-)
Accession numberICLC ATL97002
Brief descriptionSpecies: cat; Tissue: brain, fetal; Transformed by: Moloney-MSV; Original line: G355
DescriptionSpecies: cat; Tissue: brain, fetal; Transformed by: Moloney-MSV; Original line: G355
DepositorObtained from ECACC.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology glial/astrocyte
Culture conditionsMcCoy's 5A + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2.
Propertiesvirus replication; viral diagnostics; the cells can detect and assay type C retroviruses and are currently recommended by the FDA for detection of replication competent retroviruses in gene therapy products and hybridomas producing reagents for human use
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as cat with G6PD, MD.
Passage number26
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyJ Virol 1985;53:827-833 - PMID: 2983093
Virology 1982;123:139-151 - PMID: 6959413

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