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Cell line nameMG-1361
Accession numberICLC ATL97003
Brief descriptionSpecies: mouse, CD-1 transgenic; Tissue: mammary gland; Tumor: adenocarcinoma; Transformed by: rat activated neu oncogene
DescriptionSpecies: mouse, CD-1 transgenic male 5 months old; Tissue: mammary gland; Tumor: adenocarcinoma; Transformed by: rat activated neu oncogene
DepositorDr M.G. Sacco, Advanced Biomedical Technologies Institute, CNR, Milan, Italy
Reference paperBreast Cancer Res Treat 1998;47:171-180 - PMID: 9497105
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsWilliam's medium + 15% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split cultures 1:3-1:6 using trypsin/EDTA do not allow to reach confluence; seed at 3-5x104 cells/cm2; 37C, 5% CO2.
Propertiesestrogen and progestinic receptor; Neu over expression; sensitive to anti-estrogens and apoptosis inducing.
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as mouse with MD, NP
Passage number23
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyGene Ther 1998;5:388-393 - PMID: 9614559

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