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Cell line nameB3/AN
Accession numberICLC ATL97004
Brief descriptionSpecies: mouse, AKR; Tissue: thymus; Tumor: thymoma
DescriptionSpecies: mouse, AKR; Tissue: thymus; Tumor: thymoma
DepositorObtained from ECACC.
Reference paperJ Immunol Methods 1992;154:211 - DOI: 10.1016/0022-1759(92)90194-x - PMID: 1401954
Morphology and growthcontinuous culture, grown in suspension, morphology spherical
Culture conditionsDMEM + 10% HS + 2mM L-Glutamine + 1mM Na pyruvate; Maintain cultures between 3 and 9x105 cells/ml. 37C, 5-10% CO2
PropertiesLyt-2/13 negative cell line, studies of the assembly and transport of Lyt-2/13 antigen in the cell surface and definition of the regions of Lyt-2/13 interacting with corresponding antibodies and with MHC Class I molecules
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as mouse with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyImmunogenetics 1994;40:154 - DOI: 10.1007/BF00188180 - PMID: 7517914

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