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Cell line nameP388.D1 (clone 3124)
Accession numberICLC ATL97013
Brief descriptionSpecies: mouse; Tissue: lymphoid; Tumor: lymphoid neoplasm; Transformed by: methylcholanthrene-induced; Original line: P388
DescriptionSpecies: mouse; Tissue: lymphoid; Tumor: lymphoid neoplasm; derived from a tumor induced in vivo by methylcholanthrene; Original line: P388
DepositorDr A. Rubartelli, Clinical Pathology Dept, IST, Genoa, Italy
Reference paperAm J Pathol 1957;33:603
Morphology and growthcontinuous culture, grown semiadherent, morphology macrophage-like
Culture conditionsL-15 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6; seed at 2-4 x 10^4 cells/cm2; 37C
Propertiesselected for the high production of IL-1; the cells exhibit high effector activity in antibody dependent cell mediated cytotoxicity
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as mouse with MD, Pep B
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyCancer Res 1977;37:546-550 - PMID: 318922
J Immunol 1975;114:894-897 - PMID: 1167564
J Immunol 1977;119:950-954 - PMID: 894031
Commentsthe cells detach very easily; CO2 is not required

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