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Cell line nameCHO-K1
Accession numberICLC ATL98003
Brief descriptionSpecies: hamster, Chinese; Tissue: ovary
DescriptionSpecies: hamster, Chinese female adult; Tissue: ovary; Derived from: CHO
DepositorDr T. Florio, Neuroscience Unit, ABC, Genoa, Italy
Reference paperJ Exp Med 1958;108:945
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsHam's F12 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:4-1:10 using trypsin/EDTA; seed at 1-2 x 10^4 cells/cm2; 37C, 5% CO2
PropertiesThe cells have an absolute requirement for L-proline
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as hamster with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO

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