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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | CHO-K1 |
| Accession number | ICLC ATL98003 |
| Brief description | Species: hamster, Chinese; Tissue: ovary |
| Description | Species: hamster, Chinese female adult; Tissue: ovary; Derived from: CHO |
| Depositor | Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy |
| Reference paper | J Exp Med 1958;108:945 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
| Culture conditions | Ham's F12 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:4-1:10 using trypsin/EDTA; seed at 1-2 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | The cells have an absolute requirement for L-proline |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as hamster with MD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |