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Cell line namePC-12
Accession numberICLC ATL98004
Brief descriptionSpecies: rat; Tissue: adrenal; Tumor: pheochromocytoma
DescriptionSpecies: rat; Tissue: adrenal; Tumor: pheochromocytoma
DepositorDr T. Florio, Neuroscience Unit, ABC, Genoa, Italy
Reference paperProc Natl Acad Sci USA 1976;73:2424-2426
Morphology and growthcontinuous culture, grown in suspension, morphology clusters of small cells
Culture conditionsRPMI + 5% FBS + 10% HS + 2mM L-Glutamine; split 1:3-1:6; seed at 1-2 x 10^5 cells/cm2; 37C, 5% CO2
Propertiesthe cells are described to synthesize catecholamines (dopamine, norepinephrine); respond reversibly to NGF by induction of the neuronal phenotype.
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyScience 1985;229:393-395 - DOI: 10.1126/science.3839317 - PMID: 3839317
Commentsto obtain single cells, pass the suspension through a 22G syringe; use collagen-coated flasks for attachment

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