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Cell line nameAKR/12B-1
Accession numberICLC ATL98005
Brief descriptionSpecies: mouse, AKR/J; Tissue: thymus; Transformed by: X-rays
DescriptionSpecies: mouse, AKR/J; Tissue: thymus; Transformed by: X-rays
DepositorDr D. Iannelli, Immunology Dept, Faculty of Agriculture, Naples, Italy
Reference paperJ Immunol Methods 1992;154(2):211-216 - PMID: 1401954
Morphology and growthcontinuous culture, grown in suspension, morphology semi-adherent, spherical
Culture conditionsDMEM + 10% HS + 2mM L-Glutamine + 1mM Na pyruvate; maintain cultures between 3-9x10^5 cells/ml; split 1:5-1:10;37C, 5% CO2
PropertiesThe cells express Lyt-2, Lyt-3 and CD4 in the cytosol, but not on the membrane; they reexpress Lyt-2, Lyt-3 and CD4 on the membrane after sorting by magnetic particles (5 cycles)
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as mouse with MD,NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyImmunogenetics 1994;40(2):154-158 - PMID: 7517914

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