ICLC logo Cell bank
Interlab Cell Line Collection (ICLC)
On-Line Catalogue

Home - Search by name - Free text search - Search by properties

Cell line nameAKR/11-D
Accession numberICLC ATL98007
Brief descriptionSpecies: mouse, AKR/J; Tissue: thymus; Transformed by: X-rays
DescriptionSpecies: mouse, AKR/J; Tissue: thymus; Transformed by: X-rays
DepositorDr D. Iannelli, Immunology Dept, Faculty of Agriculture, Naples, Italy
Reference paperJ Immunol Methods 1992;154(2):211-216 - PMID: 1401954
Morphology and growthcontinuous culture, grown in suspension, morphology few semi-adherent cells, spherical
Culture conditionsDMEM + 10% HS + 2mM L-Glutamine + 1mM Na pyruvate; maintain cultures between 3-9 x 10^5 cells/ml; split 1:5-1:10; 37C, 5% CO2
Propertiesthe cells express Lyt-2, Lyt-3 and CD4 in the cytosol, but not on the membrane; reexpress Lyt-2 on the membrane after transfection (but not after sorting by magnetic particles)
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as mouse with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyImmunogenetics 1994;40(2):154-158 - PMID: 7517914

For any info contact Interlab Cell Line Collection
HTML by Paolo Romano