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Cell line nameJ774A.1
Accession numberICLC ATL98011
Brief descriptionSpecies: mouse, BALB/c; Tissue: monocyte-macrophage; Tumor: monocyte-macrophage tumor
DescriptionSpecies: mouse, BALB/c female; Tissue: monocyte-macrophage; Tumor: monocyte-macrophage tumor
DepositorDr A. Corsini, Institute of Pharmacological Sciences, Milan, Italy
Reference paperNature 1975;257:393-394 - DOI: 10.1038/257393a0 - PMID: 1101071
Morphology and growthcontinuous culture, grown as monolayer, morphology macrophage-like, many round cells
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA or scraper: the cells are very difficult to detach; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
Propertiesthe cells have been described to synthesize lysozyme and IL-1, and have receptors for Ig and complement
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as mouse with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyCancer Res 1977;37:546-550 - PMID: 318922
J Exp Med 1976;143:1528-1533 - DOI: 10.1084/jem.143.6.1528 - PMID: 1083890
J Immunol 1975;114:898-905 - PMID: 1089721

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