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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | J774A.1 |
| Accession number | ICLC ATL98011 |
| Brief description | Species: mouse, BALB/c; Tissue: monocyte-macrophage; Tumor: monocyte-macrophage tumor |
| Description | Species: mouse, BALB/c female; Tissue: monocyte-macrophage; Tumor: monocyte-macrophage tumor |
| Depositor | Dr A. Corsini, Institute of Pharmacological Sciences, Milan, Italy |
| Reference paper | Nature 1975;257:393-394 - DOI: 10.1038/257393a0 - PMID: 1101071 |
| Morphology and growth | continuous culture, grown as monolayer, morphology macrophage-like, many round cells |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA or scraper: the cells are very difficult to detach; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | the cells have been described to synthesize lysozyme and IL-1, and have receptors for Ig and complement |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with MD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Cancer Res 1977;37:546-550 - PMID: 318922
J Exp Med 1976;143:1528-1533 - DOI: 10.1084/jem.143.6.1528 - PMID: 1083890 J Immunol 1975;114:898-905 - PMID: 1089721 |