 |
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
|
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
| Cell line name | A8/D1 |
| Accession number | ICLC ATL98012 |
| Brief description | Species: mouse, AKR; Tissue: thymus; Tumor: thymoma; Transformed by: exposed to a dose of 500 rads from an X-ray source; Original line: AKR/1a murine T cell line |
| Description | Species: mouse, AKR; Tissue: thymus; Tumor: thymoma; the mutant Lyt-2 cells were selected out by magnetic bead separation; Original line: AKR/1a murine T cell line |
| Depositor | Obtained from ECACC |
| Reference paper | J Immunol Methods 1992;154(2):211-216 - DOI: 10.1016/0022-1759(92)90194-x - PMID: 1401954 |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast |
| Culture conditions | DMEM + 10% HS + 2mM L-Glutamine + 1mM Na pyruvate; maintain cultures between 3-9x10^5 cells/ml; split 1:5-1:10; 37C, 5% CO2 |
| Properties | useful in the study of assembly and transport of Lyt-2/3 antigen in the cell surface or to define the regions of Lyt-2/3 interacting with the corresponding antibodies and with MHC class I molecules |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with AST, MD |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Immunogenetics 1994;40(2):154-158 - DOI: 10.1007/BF00188180
- PMID: 7517914
|