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Cell line nameA8/DI
Accession numberICLC ATL98012
Brief descriptionSpecies: mouse, AKR; Tissue: thymus; Tumor: thymoma; Transformed by: exposed to a dose of 500 rads from an X-ray source; Original line: AKR/1a murine T cell line
DescriptionSpecies: mouse, AKR; Tissue: thymus; Tumor: thymoma; the mutant Lyt-2 cells were selected out by magnetic bead separation; Original line: AKR/1a murine T cell line
DepositorObtained from ECACC
Reference paperJ Immunol Methods 1992;154(2):211-216 - PMID: 1401954
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast
Culture conditionsDMEM + 10% HS + 2mM L-Glutamine + 1mM Na pyruvate; maintain cultures between 3-9x10^5 cells/ml; split 1:5-1:10; 37C, 5% CO2
Propertiesuseful in the study of assembly and transport of Lyt-2/3 antigen in the cell surface or to define the regions of Lyt-2/3 interacting with the corresponding antibodies and with MHC class I molecules
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as mouse with AST, MD
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyImmunogenetics 1994;40(2):154-158 - PMID: 7517914

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