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Cell line nameJTC-27
Accession numberICLC ATL98013
Brief descriptionSpecies: rat; Tissue: liver; Tumor: hepatoma
DescriptionSpecies: rat; Tissue: liver; Tumor: hepatoma
Depositorobtained from ECACC
Reference paperJ Biochem (Tokio) 2000;127:321-327 - DOI: 10.1093/oxfordjournals.jbchem.a022610 - PMID: 10731700
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:6-1:8 using trypsin/EDTA; seed at 1-2x10^4 cells/cm2; 37C, 5% CO2
PropertiesDrug metabolism assays, hepatocyte studies, toxicology
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as rat with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyJ Exp Med 1972;42:249

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