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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | JTC-27 |
| Accession number | ICLC ATL98013 |
| Brief description | Species: rat; Tissue: liver; Tumor: hepatoma |
| Description | Species: rat; Tissue: liver; Tumor: hepatoma |
| Depositor | obtained from ECACC |
| Reference paper | J Biochem (Tokio) 2000;127:321-327 - DOI: 10.1093/oxfordjournals.jbchem.a022610 - PMID: 10731700 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:6-1:8 using trypsin/EDTA; seed at 1-2x10^4 cells/cm2; 37C, 5% CO2 |
| Properties | Drug metabolism assays, hepatocyte studies, toxicology |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as rat with MD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | J Exp Med 1972;42:249 |