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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MH1C1 |
| Accession number | ICLC ATL98014 |
| Brief description | Species: rat; Tissue: liver; Tumor: hepatoma |
| Description | Species: rat; Tissue: liver; Tumor: hepatoma; Derived from: transplantable Morris hepatoma (n.7795, Buffalo strain) |
| Depositor | Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy |
| Reference paper | J Cell Biol 1969;40:236-247 - DOI: 10.1083/jcb.40.1.236 - PMID: 4177659 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial; the cells grow in large clusters |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-2 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | the cells synthesize and secrete serum albumin, C9, tyrosine aminotransferase; monooxigenases expression; toxicity screening; xenobiotic metabolism; drug metabolism assays; hepatocyte studies |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as rat with AST, MD |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Cell Biochem Funct 1988;6:245-250 - DOI: 10.1002/cbf.290060405
- PMID: 3191583
Exp Cell Res 1991;196:330-336 - DOI: 10.1016/0014-4827(91)90268-y - PMID: 1716586 |
| Comments | cell harvest is about 10 x 10^6 cells/25 cm2 flask |