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Interlab Cell Line Collection (ICLC)
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Cell line nameB104-1-1
Accession numberICLC ATL98015
Brief descriptionSpecies: mouse, Swiss albino; Tissue: embryo; Transfected with: DNA from rat neuroblastoma B104; Original line: NIH/3T3
DescriptionSpecies: mouse, Swiss albino; Tissue: embryo; Transfected with EcoR1 digested DNA from the rat neuroblastoma B104; Original line: NIH/3T3
DepositorDr U. Pfeffer, Molecular Biology Dept IST at ABC, Genoa, Italy
Reference paperNature 1984;312:513-516 - DOI: 10.1038/312513a0 - PMID: 6095109
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2
Propertiesthe cells contain the transforming gene neu which induces synthesis of a tumor antigen of relative molecular mass 185,000 (p185), strongly associated with the presence of neuro/glioblastoma oncogenes. The neu oncogene bears homology to erb-B and the tumor antigen p185 is serologically related to the epidermal growth factor receptor
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as mouse with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO

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