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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | Hepa 1-clc7 |
| Accession number | ICLC ATL98016 |
| Brief description | Species: mouse, C57L; Tissue: liver; Tumor: hepatoma, BW7756 |
| Description | Species: mouse, C57L; Tissue: liver; Tumor: hepatoma, BW7756; Derived from: Hepa-1cl, cloned from the Hepa-1 cell line |
| Depositor | Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy |
| Reference paper | Dev Biol 1973;35:83-96 - DOI: 10.1016/0012-1606(73)90008-0 - PMID: 4362668 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | the cells possess the aryl hydrocarbon (Ah) receptor and are highly inducible for cytochrome P4501A1; drug metabolism assays |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with MD, Pep B |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Biochimie 1991;73:61-66 - DOI: 10.1016/0300-9084(91)90075-c
- PMID: 1851644
Proc Natl Acad Sci USA 1979;76:373-376 - DOI: 10.1073/pnas.76.1.373 - PMID: 106390 Science 1980;209:497-499 - DOI: 10.1126/science.6248960 - PMID: 6248960 |