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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | L1210 |
| Accession number | ICLC ATL99002 |
| Brief description | Species: mouse, DBA/2; Tissue: ascitic fluid; Tumor: leukemia, lymphocytic; Transformed by: tumor induced in vivo with methylcholanthrene |
| Description | Species: mouse, DBA/2 female; Tissue: ascitic fluid; Tumor: leukemia, lymphocytic; tumor induced in vivo by skin paintings with 0.2% methylcholanthrene |
| Depositor | Dr A. Pessina, Cell Culture Laboratory, Institute of Microbiology, University of Milan, Italy |
| Reference paper | J Natl Cancer Inst 1949;10:179-192 |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast-like |
| Culture conditions | McCoy's 5A + 10% FBS + 2mM L-Glutamine; maintain cultures between 1-5 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2 |
| Properties | screening of chemical agents; antitumor activity testing in cancer chemotherapy screening studies |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with MD, NP |
| Passage number | 285 |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |