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Cell line nameL1210
Accession numberICLC ATL99002
Brief descriptionSpecies: mouse, DBA/2; Tissue: ascitic fluid; Tumor: leukemia, lymphocytic; Transformed by: tumor induced in vivo with methylcholanthrene
DescriptionSpecies: mouse, DBA/2 female; Tissue: ascitic fluid; Tumor: leukemia, lymphocytic; tumor induced in vivo by skin paintings with 0.2% methylcholanthrene
DepositorDr A. Pessina, Cell Culture Laboratory, Institute of Microbiology, University of Milan, Italy
Reference paperJ Natl Cancer Inst 1949;10:179-192
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast-like
Culture conditionsMcCoy's 5A + 10% FBS + 2mM L-Glutamine; maintain cultures between 1-5 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2
Propertiesscreening of chemical agents; antitumor activity testing in cancer chemotherapy screening studies
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as mouse with MD, NP
Passage number285
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO

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