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On-Line Catalogue

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Cell line nameWEHI-3B
Accession numberICLC ATL99003
Brief descriptionSpecies: mouse, BALB/c; Tissue: monocyte; Tumor: leukemia, myelomonocytic
DescriptionSpecies: mouse, BALB/c; Tissue: monocyte; Tumor: leukemia, myelomonocytic
DepositorDr A. Pessina, Cell Culture Laboratory, Institute of Microbiology, University of Milan, Italy
Reference paperJ Natl Cancer Inst 1969;43:963
Morphology and growthcontinuous culture, grown in suspension, morphology small, round, single cells
Culture conditionsMcCoy's 5A + 10% FBS + 2mM L-Glutamine; maintain cultures between 1-5 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2
Propertiesthe cells produce IL-3
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as mouse with MD, Pep B
Passage number273
Freezing mediumCulture medium + 50% FBS + 10% DMSO

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