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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SR-4987 |
| Accession number | ICLC ATL99005 |
| Brief description | Species: mouse, BDF/1; Tissue: bone marrow, stromal cells; Transformed by: murine leukemia virus |
| Description | Species: mouse, BDF/1 female 5 months old; Tissue: bone marrow, stromal cells; Transformed by: murine leukemia virus |
| Depositor | Dr A. Pessina, Cell Culture Laboratory, Institute of Microbiology, University of Milan, Italy |
| Reference paper | Cytotechnology 1992;8:93-102 - DOI: 10.1007/BF02525491 - PMID: 1382506 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
| Culture conditions | McCoy's 5A + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | the cells are reported to produce macrophage-colony stimulating factor (M-CSF) and are highly sensitive to fibroblast growth factor (FGF) |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with MD, Pep B |
| Passage number | 197 |
| Tumorigenicity | tumorigenic in syngeneic animals |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Exp Hematol 1997;25:536-541 - PMID: 9197333
Methods Mol Cell Biol 1995;5:230-236 |