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Cell line nameF9
Accession numberICLC ATL99006
Brief descriptionSpecies: mouse, 129; Tissue: testis; Tumor: teratocarcinoma
DescriptionSpecies: mouse, 129; Tissue: testis; Tumor: teratocarcinoma
DepositorDr S. Astigiano, Transgenic Unit, IST at ABC, Genoa, Italy
Reference paperProc Natl Acad Sci USA 1973;70:3899-3903 - PMID: 4521215
Morphology and growthcontinuous culture, grown as monolayer, morphology undifferentiated
Culture conditionsDMEM (4.5 g/L glucose) + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4x10^4; 37C, 5% CO2
PropertiesThe cells undergo very limited differentiation under normal culture conditions, but can be induced to differentiate into a) parietal endoderm in the presence of retinoic acid (RA) and dibutyryl cyclic AMP; b) visceral endoderm when cultured in suspension as aggregates and treated with RA. Differentiation markers are: tissue type plasminogen activator (tPA) for parietal endoderm cells; alpha fetoprotein (AFP) and urokinase plasminogen activator (uPA) for visceral endoderm cells. In addition, both cell types lose the stage specific embryonic antigen-1 (SSEA-1), and synthesize large amounts of type IV collagen, laminin and fibronectin.
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as mouse with MD, NP
Passage number6
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyCell 1978;15:393-403 - PMID: 214238
Commentspassage 6 after in vivo passage; split every two days; leave the cells with trypsin for one or two minutes at room temperature; the flasks must be presoaked with 0.1% gelatin for 10 minutes

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